Question 1

Which step involves transformation?

Accepted Answer

A)  Target DNA is removed from cells and isolated.
B)  The cloning host takes up a plasmid.
C)  Target DNA and plasmid are treated with the same restriction endonuclease.
D)  The desired protein is produced by the cloning host.
E)  The gene is amplified by multiplication of the cloning host.F) C) and D)
G) A) and C)

Question 2

Which PCR step synthesizes complementary DNA strands?

Accepted Answer

A)  Add DNA polymerase and nucleotides at 72°C.
B)  Cool DNA to between 50°C and 65°C.
C)  Add primers.
D)  Heat target DNA to 94°C.
E)  Repeat the cycle of heating and cooling.F) A) and E)
G) B) and E)

Question 3

All of the following are characteristics that make plasmids good cloning vectors, except

Accepted Answer

A)  they are small.
B)  they are well characterized.
C)  they can be transferred into appropriate host cells.
D)  they can carry genetic markers, such as antibiotic resistance genes.
E)  they can accept a relatively large amount of foreign DNA.F) B) and E)
G) B) and C)

Question 4

Some genes in bacteria are repressed by antisense RNA that binds to them.

Accepted Answer

A) True 
 B)False

Question 5

To be of value, DNA sequences targeted for analysis for DNA profiling need to be located in coding regions of the genome.

Accepted Answer

A) True 
 B)False

Question 6

Which of the following drugs is/are produced by genetic engineering and approved for human use?

Accepted Answer

A)  Human growth hormone
B)  Factor VIII
C)  insulin
D)  tissue plasminogen activator
E)  All of these choices are correct.F) C) and D)
G) C) and E)

Question 7

The _____ blot method analyzes DNA, while the _____ method analyzes RNA.

Accepted Answer

The answer of The _____ blot method analyzes DNA, while...

Question 8

VNTRs and microsatellite polymorphisms are genetic markers seen in DNA fingerprinting.

Accepted Answer

A) True 
 B)False

Question 9

Which of the following methods determines which genes are actively transcribed in a cell under a variety of conditions?

Accepted Answer

A)  DNA fingerprinting
B)  microarray analysis
C)  gene mapping
D)  Western blot
E)  antisense therapyF) A) and B)
G) B) and C)

Question 10

Describe what is meant by a DNA fingerprint and how it is used in forensic science.

Accepted Answer

A DNA fingerprint is a unique pattern of...

Question 11

EcoRI and HindIII are

Accepted Answer

A)  palindromes.
B)  reverse transcriptases.
C)  restriction endonucleases.
D)  ligases.
E)  DNA polymerases.F) A) and B)
G) B) and C)

Question 12

When DNA is heated to just below boiling (90°C-95°C) ,

Accepted Answer

A)  the sugar phosphate backbone of the DNA strands breaks.
B)  the sugar, phosphate, and base come apart in each nucleotide.
C)  the helix unwinds but hydrogen bonds between the bases remain intact.
D)  the two DNA strands separate completely.
E)  nothing happens until the boiling point is reached (100o
C)  .F) All of the above
G) C) and D)

Question 13

The size of DNA is often given in the number of _____ that it contains.

Accepted Answer

base pairs...

Question 14

Compare and contrast recombinant DNA and gene therapy.

Accepted Answer

Recombinant DNA and gene therapy are bot...

Question 15

Labeled, known, short stretches of DNA used to detect a specific sequence of nucleotides in a mixture are

Accepted Answer

A)  genetic engineering.
B)  biotechnology.
C)  recombinant DNA.
D)  gel electrophoresis.
E)  gene probes.F) A) and E)
G) A) and D)

Question 16

All of the following are correct about DNA fingerprinting, except

Accepted Answer

A)  it displays the unique pattern of an individual's DNA fragments.
B)  it can be used to analyze evidence when only minute amounts of DNA are found.
C)  it cannot be used on specimens older than five years.
D)  it is routine to use it as trial evidence in the U.S.
E)  it can be used on hair and saliva.F) None of the above
G) D) and E)

Question 17

Both DNA sequencing and polymerase chain reactions require special __________ to initiate synthesis of a new DNA molecule.

Accepted Answer

A)  RNA promoters
B)  fluorescent nucleotides
C)  primers
D)  thermophilic polymerases
E)  endonucleasesF) A) and C)
G) B) and D)

Question 18

In recombinant DNA technique, an enzyme called _____ is needed to seal the sticky ends of genes into plasmids or chromosomes.

Accepted Answer

The answer of In recombinant DNA technique, an enzyme called...

Question 19

Draw and label the specific sequence of procedures, reagents, and materials needed to obtain a human protein from E.coli cells growing in a lab culture.

Accepted Answer

1. Start by growing E. coli cells in a l...

Question 20

Which of the following is often used in forensic science to distinguish one sequence of DNA from another by comparing the sequence of the strands at specific loci?

Accepted Answer

A)  cloning
B)  gene therapy
C)  antisense therapeutic
D)  DNA fingerprintingE) A) and B)
F) A) and C)

Exam 10: Genetic Engineering: a Revolution in Molecular Biology

Some genes in bacteria are repressed by antisense RNA that binds to them.

Correct AnswerverifiedShow Answer

In recombinant DNA technique, an enzyme called _____ is needed to seal the sticky ends of genes into plasmids or chromosomes.

Correct AnswerverifiedShow Answer

When DNA is heated to just below boiling (90°C-95°C) ,

Correct AnswerverifiedShow Answer

EcoRI and HindIII are

Correct AnswerverifiedShow Answer

Which of the following methods determines which genes are actively transcribed in a cell under a variety of conditions?

Correct AnswerverifiedShow Answer

Which PCR step synthesizes complementary DNA strands?

Correct AnswerverifiedShow Answer

Compare and contrast recombinant DNA and gene therapy.

Correct AnswerAnswered by ExamLex AIRecombinant DNA and gene therapy are bot...View AnswerShow Answer

Which step involves transformation?

Correct AnswerverifiedShow Answer

Which of the following is often used in forensic science to distinguish one sequence of DNA from another by comparing the sequence of the strands at specific loci?

Correct AnswerverifiedShow Answer

Describe what is meant by a DNA fingerprint and how it is used in forensic science.

Correct AnswerAnswered by ExamLex AIA DNA fingerprint is a unique pattern of...View AnswerShow Answer

All of the following are characteristics that make plasmids good cloning vectors, except

Correct AnswerverifiedShow Answer

To be of value, DNA sequences targeted for analysis for DNA profiling need to be located in coding regions of the genome.

Correct AnswerverifiedShow Answer

Which of the following drugs is/are produced by genetic engineering and approved for human use?

Correct AnswerverifiedShow Answer

Draw and label the specific sequence of procedures, reagents, and materials needed to obtain a human protein from E.coli cells growing in a lab culture.

Correct AnswerAnswered by ExamLex AI1. Start by growing E. coli cells in a l...View AnswerShow Answer

Both DNA sequencing and polymerase chain reactions require special __________ to initiate synthesis of a new DNA molecule.

Correct AnswerverifiedShow Answer

All of the following are correct about DNA fingerprinting, except

Correct AnswerverifiedShow Answer

Labeled, known, short stretches of DNA used to detect a specific sequence of nucleotides in a mixture are

Correct AnswerverifiedShow Answer

The _____ blot method analyzes DNA, while the _____ method analyzes RNA.

Correct AnswerverifiedShow Answer

The size of DNA is often given in the number of _____ that it contains.

Correct Answerverifiedbase pairs...View AnswerShow Answer

VNTRs and microsatellite polymorphisms are genetic markers seen in DNA fingerprinting.

Correct AnswerverifiedShow Answer

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Answered by ExamLex AI
Recombinant DNA and gene therapy are bot...
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Answered by ExamLex AI
A DNA fingerprint is a unique pattern of...
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Correct Answer
Answered by ExamLex AI
1. Start by growing E. coli cells in a l...
View Answer

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The _ blot method analyzes DNA, while the _ method analyzes RNA.

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base pairs...
View Answer

Correct Answer
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